Epididymal sperm harvest

Uploader: Achilles 21 days ago  
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Retrograde ejaculation in a stallion. Harvesting sperm from a man with a testicular problem is much more difficult and often takes several hours. The vas deferens was clamped to avoid loss of semen during the 5-minute transit to the laboratory. Viable sperm cells were successfully recovered with both types of epididymal collection. Tricyclic antidepressants, such as imipramine, have been used as therapy for urospermia in stallions in an effort to increase tone of the bladder sphincter Following the unsuccessful ejaculation attempts with the mm ram probes and the inability to place the mm bull probe, a 48 cm long, mm diameter probe with 6 ring electrodes was used on 2 of the larger ponies in an attempt to increase stimulation of pelvic nerves. The number of sperm in the effluent from filter samples and in the control samples was determined by counting in a hemocytometer and these results were used to calculate the percentage of cells passing through the filter for each treatment.

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